Wente, Moritz N. The prostate stem cell antigen PSCA is a glycosyl-phosphatidyl-inositol GPI -linked cell surface antigen expressed in normal prostate and overexpressed in the majority of prostate cancers and correlates with tumor grade and disease stage. Because PSCA has been described to be up-regulated in pancreatic cancer, the purpose was to evaluate the expression of PSCA in human pancreatic cancer. Furthermore, the therapeutic efficacy of a monoclonal anti-PSCA antibody in an in vivo pancreatic cancer model was detd.
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The expression of PSCA in human pancreatic cancer tissues was detd. PSCA was strongly up-regulated in human pancreatic cancer compared with chronic pancreatitis and normal pancreas. Treatment with 1G8 significantly reduced tumor growth initiation in an in vivo pancreatic cancer xenograft model. These results show a potential therapeutic role for anti-PSCA antibodies in the treatment of pancreatic cancer. Furthermore, PSCA might serve as a novel marker in the diagnosis of pancreatic cancer.
Theragnostics polymer nanoparticles NPs loaded simultaneously with anticancer drug docetaxel Dtxl and superparamagnetic iron oxide SPIO nanocrystals were developed for both cancer therapy and ultrasensitive MRI. These multifunctional polymer vesicles were formed by carboxy-terminated poly lactic-co-glycolic acid using a single emulsion evapn.
The active tumor-targeting single chain prostate stem cell antigen antibodies scAbPSCA were conjugated on the surface of polymer vesicles by using functional poly ethylene glycol. The diam.
Novel nanoparticles deliver CRISPR gene editing tools into the cell with much higher efficiency
Vibration simple magnetometer and X-ray diffraction proved that the superparamagnetic behavior of SPIO was not changed during NPs formation and modification. The NPs exhibited a triphasic drug release pattern in vitro over 30 days. Enhanced cellular uptake ability and antiproliferative effect of the targeted NPs in prostate cancer PC3 cell line by using the confocal laser scanning microscopy and cytotoxicity assay were obsd. Therefore, these stable and tumor-targeting polymer NPs could be promising multifunctional vesicles for simultaneous targeting imaging, drug delivery and real time monitoring of therapeutic effect.
Aberrantly expressed c-Met, the receptor for hepatocyte growth factor HGF , has been implicated in human lung cancer as well as malignancy, metastasis and drug-resistance in other human cancers. Thus, this mol. Targeting delivery of compd. In this study, we utilized phage display to identify human single chain variable fragment scFv antibodies that specifically bound to c-Met protein. The anti-c-Met scFvs selectively bound to and internalized in several lung cancer cell lines expressing c-Met.
Recent advances in the development of gene delivery systems | Biomaterials Research | Full Text
Conjugation of anti-c-Met scFv with PEGylated liposomes enabled the efficient delivery of doxorubicin into cancer cells where it exerted cytotoxic activity by inducing apoptosis pathway. In a tumor xenograft model, anti-c-Met immunoliposome was found to selectively increase tumor accumulation of a chemotherapeutic drug and enhance its antitumor activity. Taken together, our results suggest that anti-c-Met scFv-mediated drug delivery systems show great promise in tumor-targeted therapy and imaging. Glia , 39 2 , ISSN: Highly migratory neuroectodermal cells share a common embryonic origin with cells of the central nervous system CNS.
They include enteric, parasympathetic, sympathoadrenal, and sensory neurons of the peripheral nervous system, Schwann cells, melanocytes, endocrine cells, and cells forming connective tissue of the face and neck. Because of their common embryologic origin, these cells and the tumors that derive from them can share genetic and antigenic phenotypes with gliomas, tumors derived from CNS glia.
We recently discovered that chlorotoxin ClTx , a 4-kD peptide purified from Leiurus quinquestriatus scorpion, is a highly specific marker for glioma cells in biopsy tissues Soroceanu et al. Cancer Res , that can target tumors in animal models. We report on the specificity of ClTx as a marker for tumors of neuroectodermal origin that include peripheral neuroectodermal tumors PNET and gliomas.
Specifically, we histochemically stained frozen and paraffin tissue sections of human biopsy tissues from patients with a synthetically manufactured and biologically active ClTx bearing an N-terminal biotin. By comparison, 32 biopsies of uninvolved brain used for comparison were largely ClTx-negative, with only a few isolated reactive astrocytes showing some ClTx binding.
These include medulloblastomas 4 of 4 , neuroblastomas 6 of 7 , ganglioneuromas 4 of 4 , melanomas 7 of 7 , adrenal pheochromocytomas 5 of 6 , primitive PNET 1 , small cell lung carcinoma 2 of 3 , and Ewing's sarcoma 2 of 2. Under identical staining conditions, normal tissues from brain, skin, kidney, and lung were consistently negative for ClTx. These results suggest that chlorotoxin is a reliable and specific histopathological marker for tumors of neuroectodermal origin and that chlorotoxin derivatives with cytolytic activity may have therapeutic potential for these cancers.
American Association for Cancer Research. Nanoparticle-based platforms have drawn considerable attention for their potential effect on oncol. However, their in vivo application is challenged by insufficient accumulation and retention within tumors due to limited specificity to the target, and an inability to traverse biol.
Here, we present a nanoprobe that shows an ability to cross the blood-brain barrier and specifically target brain tumors in a genetically engineered mouse model, as established through in vivo magnetic resonance and biophotonic imaging, and histol. The nanoprobe is comprised of an iron oxide nanoparticle coated with biocompatible polyethylene glycol-grafted chitosan copolymer, to which a tumor-targeting agent, chlorotoxin, and a near-IR fluorophore are conjugated.
The nanoprobe shows an innocuous toxicity profile and sustained retention in tumors. With the versatile affinity of the targeting ligand and the flexible conjugation chem. Iron oxide nanoparticles offer a feasible tool for combined imaging and delivery of small interfering RNA siRNA to tumors, stimulating active interest in exploring different imaging and delivery platforms suitable for detection by a variety of modalities. Nanodrug uptake by human breast adenocarcinoma cells resulted in a significant downregulation of BIRC5.
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Following i. Furthermore, MRI could be used to quant. Our strategy permits the simultaneous tumor-specific delivery of siRNA to tumors and the imaging of the delivery process. More generally, it illustrates the potential to apply this approach to many human cancer studies, including for basic tumor biol.
Cancer Res; 70 19 ; Tumor selective reactivity of a monoclonal antibody prepared against a recombinant peptide derived from the DF3 human breast carcinoma-associated antigen Cancer Res. Perey, Lucien; Hayes, Daniel F. The DF3 antigen is a member of a family of high mol. Characterization of this MAb demonstrated reactivity with immature precursors of DF3 antigen and not with the secreted form.
These findings are in contrast to those obtained with MAb DF3, a previously described antibody with predominant reactivity against the mature glycoprotein. Competition studies with synthetic peptides indicated that the proline in this domain is involved in both epitopes, while the potential glycosylation sites at threonine and serine may contribute to the differential reactivity of MAbs DF3 and DF3-P. Apparently, both antibodies react with a similar epitope that is modified by the presence of carbohydrate moieties.
While MAb DF3-P reacts with formalin-fixed sections of breast carcinomas, this antibody exhibits little if any reactivity with normal mammary epithelium. Selective expression of the DF3-P epitope in malignant breast cells may be useful in identifying this transformed phenotype. Comparative evaluation of novel biodegradable nanoparticles for the drug targeting to breast cancer cells Eur.
Mattu, C. Nanomedicine formulations such as biodegradable nanoparticles nps and liposomes offer several advantages over traditional routes of administration: due to their small size, nanocarriers are able to selectively accumulate inside tumors or inflammatory tissues, resulting in improved drug efficacy and reduced side effects.
Bioresponse Inspired Nanomaterials for Targeted Drug and Gene Delivery
To further augment targeting ability of nanoparticles towards tumor cells, specific ligands or antibodies that selectively recognize biomarkers over-expressed on cancer cells, can be attached to the surface either by chem. Nps were prepd. Polyurethanes were introduced as matrix-forming materials for nanoparticles due to their high chem.
All nps exhibited a small size and neg. Paclitaxel, a model anti-neoplastic drug, was encapsulated inside all nps, and release profiles and cytotoxicity on SK-BR-3 cells were also assessed. Interestingly, PUR nps were superior to com. Results obtained warrants further investigation on the application of these PUR nps for controlled drug delivery and targeting.
International journal of nanomedicine , 8 , ISSN:. Transmission electron microscopy revealed internalization of the receptor-targeted nanoparticles by the targeted cancer cells. The developed IONPs showed a long blood circulation time serum half-life In vitro and in vivo toxicity of CdTe nanoparticles J. American Scientific Publishers. Cadmium telluride CdTe nanoparticles exhibit strong and stable fluorescence that is attractive for many applications such as biol.